Journal of Clinical Medicine Research, ISSN 1918-3003 print, 1918-3011 online, Open Access
Article copyright, the authors; Journal compilation copyright, J Clin Med Res and Elmer Press Inc
Journal website https://jocmr.elmerjournals.com

Original Article

Volume 16, Number 11, November 2024, pages 536-546

Downregulation of miR-25-3p and Its Impact on PTAFR and IGF2BP3 Expression in Type 2 Diabetes Mellitus: Implications for Biomarker Discovery and Disease Pathogenesis

Figures

↓  Figure 1. Principal component analysis (PCA) mapping showed the differential miRNA expression between DM and NDM. DM: diabetes mellitus; miRNA: microRNA; NDM: non-diabetes mellitus.
Figure 1.
↓  Figure 2. Hierarchical clustering showed the differential miRNA expression between DM and NDM. DM: diabetes mellitus; miRNA: microRNA; NDM: non-diabetes mellitus.
Figure 2.
↓  Figure 3. RNA secondary structure of miR-25-3p.
Figure 3.
↓  Figure 4. miR-25-3p expression in vein tissue from the human miRNA tissue atlas. miRNA: microRNA.
Figure 4.
↓  Figure 5. miR-25-3p downregulated in DM patients. DM: diabetes mellitus.
Figure 5.
↓  Figure 6. PTAFR and IGF2BP3 upregulated in DM patients. DM: diabetes mellitus; IGF2BP3: insulin-like growth factor 2 mRNA binding protein 3; PTAFR: platelet-activating factor receptor.
Figure 6.

Tables

↓  Table 1. Characteristics of Patients With Type 2 Diabetes Mellitus (DM) and Patients Without Type 2 Diabetes Mellitus (NDM)
 
Sample ID Sex Age (years) FBS (mg/dL) HbA1c (%)
FBS: fasting blood sugar; HbA1c: hemoglobin A1c.
DM 1 M 33 270 13.8
DM 2 F 72 182 11.9
DM 3 M 83 205 11.7
DM 4 F 41 146 11.7
DM 5 F 40 145 11.3
DM 6 M 40 209 9.8
DM 7 F 62 191 9.8
DM 8 F 66 126 9.8
DM 9 F 62 211 9.2
DM 10 F 49 156 8.6
NDM 11 M 46 95 4.4
NDM 12 F 46 79 4.7
NDM 13 F 33 81 5.3
NDM 14 F 40 84 5.4
NDM 15 F 47 90 5.4
NDM 16 M 43 91 5.4
NDM 17 M 54 82 5.5
NDM 18 M 26 91 5.5
NDM 19 F 59 99 5.5
NDM 20 M 56 80 5.6

 

↓  Table 2. In-House and Third-Party Resources Are Included in miRTargetLink 2.0 to Select the Potential miRNA Expression Involved in the Pathogenesis of Diabetes
 
Database Task Own/third-party Type Version
miRNA: microRNA.
miRTarBase miRNA target database Third-party Database 8.0 (2020)
miRDB miRNA target database Third-party Database 6.0

 

↓  Table 3. Ten Downregulated and Six Upregulated miRNAs Are Involved in the Pathogenesis of Diabetes
 
No. miRNA Glucose metabolism Platelet reactivity Insulin resistance
miRNA: microRNA.
Ten downregulated
  1. miR-92a-3p UGP2 PTAFR IGF2
INSIG1
  2. miR-140-3p - - -
  3. let-7b-5p UGCG PTAFR IGF2BP1
IGF1R
IGF2BP3
  4. miR-25-3p UGP2 PTAFR IGF2BP3
INSIG1
  5. miR-425-5p SOGA1 PAFAH1B1 IGF1R
  6. miR-93-5p UGCG
SOGA1		 PAFAH1B2
PDGFRB
PAFAH1B1		 IGF2BP1
  7. miR-6807-5p SOGA1 - -
  8. miR-103a-3p SOGA1 PAFAH1B2 -
  9. miR-24-3p UGCG PAFAH1B2
PDGFRB		 INSIG1
  10. miR-210-3p - - IGF2
Six upregulated
  1. miR-4428 SOGA1 - -
  2. miR-4253 - PAFAH1B2 -
  3. miR-150-3p - PDGFRB
PAFAH1B1		 -
  4. miR-5100 SOGA1 - -
  5. miR-939-5p SOGA1 - IGF2
  6. miR-6824-5p - - -